Chlamydomonas Research Projects

1. Van Dijk, K., Marley, K.E., Jeong, B., Xu, J., Hesson, J., Cerny, R.L., Waterborg, J.H. and Cerutti, H. Monomethyl histone H3 lysine 4 as an epigenetic mark for silenced euchromatin in Chlamydomonas The Plant Cell 17: 2439-2453, 2005.
2. Waterborg, J.H., Robertson, A.J., Tatar, D.L., Borza, C.M. and Davie, J.R. Histones of Chlamydomonas reinhardtii: Synthesis, Acetylation and Methylation Plant Physiol. 109: 393-407, 1995.
3. Waterborg, J.H. Dynamics of Histone Acetylation in Chlamydomonas reinhardtii. J. Biol. Chem. 273: 27602–27609, 1998.

A slide show presentation at the 19th West Coast Chromatin and Chromosomes Meeting, Asilomar CA, December 1997:
1. Title Slide: Dynamics of Histone Acetylation in Chlamydomonas
2. HPLC fractionation of histones, labeled with tritiated acetate
3. AUT gel of histone H3: Coomassie and fluorograph
4. AUT gel of histone H4: Coomassie and fluorograph
5. Dynamic acetylation levels in all core histones
6. Rapid acetate incorporation with exhaustion of label
7. Pulse-chase of acetate label in histones
8. Levels of steady-state and dynamic acetylation in H3, H4, H2B
9. Fluorography and Coomassie data on histone H3 acetylation levels
10. Dynamics of each acetylation level in histone H3
11. Dynamics of each acetylation level in histone H4
12. Conclusion: What have we learned until now about the Dynamics of Histone Acetylation in Chlamydomonas ?
    Follow-up research, presented at the 20th West Coast Chromatin and Chromosomes Meeting, Asilomar CA, December 1998; published as reference #2:
13. Turnover rates of acetylation on histones of Chlamydomonas
14. Turnover rates of acetylation per acetylation level in histone H3
15. Fraction of acetylated histone H3 subject to turnover of acetylation
16. Fraction of acetylated histone H4 subject to turnover of acetylation
17. Trichostatin induces hyperacetylation of histone H3
18. Dynamics of histone H3 hyperacetylation
19. Trichostatin induces hyperacetylation of histone H4
20. Dynamics of histone H4 hyperacetylation